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NEW! Brilliant Violet™ Conjugates

Jackson Immunoresearch ha sviluppato una nuova gamma di anticorpi secondari coniugati per le analisi in fluorescenza:

Brilliant Violet 421 and 480 Secondary Antibody Conjugates

bv_hero1.jpg
 Flyer informativo>>>

Nuovi fluorocromi per multiple labelling nella regione UV / Vis: Brilliant Violet 421 e Brilliant Violet 480 coniugati con anticorpi secondari.

CAMPI DI APPLICAZIONI PRINCIPALI:

1) Se combinati con Alexa Fluor® 488, Rhodamine-Red-X e Alexa Fluor® 647 è possibile un multilabelling a 5 fluorocromi.

2) Usando in alternativa al DAPI (emissione nella regione blu) il DRAQ5™ (che ha emissione rossa) si libera la regione viola-blu dello spettro per poter accogliere i due coloranti Brilliant Violet. Si può così evidenziare il nucleo e ottenere una colorazione a 4 colori: Brilliant Violet™ 421, Brilliant Violet™ 480, Alexa Fluor® 488 e Rhodamine Red™-X.

frequenze.jpg
Eccitazione ed emissione:bv-spectra.jpg
Curve di eccitazione e di emissione per BV421, BV480, Alexa Fluor® 488, Rhodamin-Red-X, e Alexa Fluor® 657.
 
Colorazioni Brilliant Violet:
Le catene polimeriche Brilliant Violet possono essere considerate come un insieme di segmenti ottici, ciascuno con la capacità di assorbire la luce ed emettere il segnale di fluorescenza. Ciò si traduce in materiali che hanno un segnale di fluorescenza per una migliore risoluzione e sensibilità.
 bv-absorption.jpg Polimeri di base per la raccolta della luce hanno più segmenti ottici in grado di emettere per rilevare la fluorescenza.
Set filtri raccomandato: recommended_filter_sets.jpg

Spectral properties:spectral_properties.jpg

 Lista dei nuovi anticorpi secondari coniugati Brilliant Violet 421 and 480:
Codice Descrizione
005-670-003 Brilliant Violet™ 421-ChromPure Goat IgG, whole molecule
005-680-003 Brilliant Violet™ 480-ChromPure Goat IgG, whole molecule
017-670-003 Brilliant Violet™ 421-ChromPure Donkey IgG, whole molecule
017-680-003 Brilliant Violet™ 480-ChromPure Donkey IgG, whole molecule
111-675-144 Brilliant Violet™ 421-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot)
111-685-144 Brilliant Violet™ 480-AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot)
112-675-071 Brilliant Violet™ 421-AffiniPure Goat Anti-Rat IgG, Fcγ Fragment Specific (min X Hu,Bov,Hrs Sr Prot)
112-675-075 Brilliant Violet™ 421-AffiniPure Goat Anti-Rat IgM, µ Chain Specific (min X Hu,Bov,Hrs Sr Prot)
112-675-143 Brilliant Violet™ 421-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs,Rb Sr Prot)
112-675-167 Brilliant Violet™ 421-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs,Ms,Rb Sr Prot) 
112-685-071 Brilliant Violet™ 480-AffiniPure Goat Anti-Rat IgG, Fcγ Fragment Specific (min X Hu,Bov,Hrs Sr Prot)
112-685-075 Brilliant Violet™ 480-AffiniPure Goat Anti-Rat IgM, µ Chain Specific (min X Hu,Bov,Hrs Sr Prot)
112-685-143 Brilliant Violet™ 480-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs,Rb Sr Prot)
112-685-167 Brilliant Violet™ 480-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs,Ms,Rb Sr Prot) 
115-675-071 Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG, Fcγ Fragment Specific (min x Hu,Bov,Hrs Sr Prot)
115-675-075 Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgM, µ Chain Specific (min X Hu,Bov,Hrs Sr Prot)
115-675-146 Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot)
115-675-166 Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Rat Sr Prot)
115-675-205 Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific (min X Hu,Bov,Rb Sr Prot)
115-675-206 Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific (min X Hu,Bov,Rb Sr Prot)
115-675-207 Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific (min X Hu,Bov,Rb Sr Prot)
115-675-209 Brilliant Violet™ 421-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific (min X Hu,Bov,Rb Sr Prot)
115-685-071 Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG, Fcγ Fragment Specific (min x Hu,Bov,Hrs Sr Prot)
115-685-075 Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgM, µ Chain Specific (min X Hu,Bov,Hrs Sr Prot)
115-685-146 Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot)
115-685-166 Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Rat Sr Prot)
115-685-205 Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 Specific (min X Hu,Bov,Rb Sr Prot)
115-685-206 Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a Specific (min X Hu,Bov,Rb Sr Prot)
115-685-207 Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific (min X Hu,Bov,Rb Sr Prot)
115-685-209 Brilliant Violet™ 480-AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 Specific (min X Hu,Bov,Rb Sr Prot)
703-675-155 Brilliant Violet™ 421-AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (min X Bov,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
703-685-155 Brilliant Violet™ 480-AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (min X Bov,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
705-675-147 Brilliant Violet™ 421-AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
705-685-147 Brilliant Violet™ 480-AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
706-675-148 Brilliant Violet™ 421-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
706-685-148 Brilliant Violet™ 480-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
709-675-149 Brilliant Violet™ 421-AffiniPure Donkey Anti-Human IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Ms,Rb,Rat,Shp Sr Prot)
709-685-149 Brilliant Violet™ 480-AffiniPure Donkey Anti-Human IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Ms,Rb,Rat,Shp Sr Prot)
711-675-152 Brilliant Violet™ 421-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)
711-685-152 Brilliant Violet™ 480-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)
712-675-150 Brilliant Violet™ 421-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
712-675-153 Brilliant Violet™ 421-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot)
712-685-150 Brilliant Violet™ 480-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
712-685-153 Brilliant Violet™ 480-AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rb,Shp Sr Prot)
713-675-147 Brilliant Violet™ 421-AffiniPure Donkey Anti-Sheep IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
713-685-147 Brilliant Violet™ 480-AffiniPure Donkey Anti-Sheep IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
715-675-150 Brilliant Violet™ 421-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
715-675-151 Brilliant Violet™ 421-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot)
715-685-150 Brilliant Violet™ 480-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot)
715-685-151 Brilliant Violet™ 480-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot)
109-675-098
Brilliant Violet™ 421 AffiniPure Goat Anti-Human IgG, Fcγ Fragment Specific (min X Bov, Hrs, Ms Sr Prot)
109-685-098 Brilliant Violet™ 480 AffiniPure Goat Anti-Human IgG, Fcγ Fragment Specific (min X Bov, Hrs, Ms Sr Prot)
 
 Brilliant Violet™ is a trademark of Sirigen Inc., a Becton, Dickinson and Company affiliate.
 
 PER ULTERIORI INFORMAZIONI CONTATTACI.
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