LiStarFish - ANTICORPI PRIMARI E SECONDARI - Prodotti per la ricerca medica

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Antibodies to Loading Controls
Beta Actina, Tubulina e GAPDH
   
 GAMMA COMPLETA ANTICORPI PRIMARI Clicca qui
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Western  blot analysis  can  be  used  for  (semi-)quantitative  detection  of target  proteins,  especially  concerning  experimental  procedures  which lead to altered expression levels of the target protein.
To compare the effect of different treatments on the target protein a method to standardize the amount of total protein loaded per lane and to confirm that equal amounts were loaded in each lane of the gel is needed. 
For this purpose so called ‘house keeping’ proteins are selected as controls. The loading control ideally should not show an altered expression level during the course of the experiment so one has to carefully choose the  appropriate  loading  control  for  each  experimental  setup.  Proteins selected  as  loading  controls  are  of ten  of  fundamental  importance  in  maintaining basic cellular functions.
 
 beta_act-gapdh-tub.jpg
   
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